LEAF CAROTENES

Enzymatic Synthesis of Carotenes

The pathway of carotene biosynthesis from isopentenyl pyrophosphate in tomato fruit plastids has been established, except for minor details, through the conversion of radioactive sub— strates and intermediates to acyclic and cyclic carotenes in cell—free systems. After the pathway of carotene biosynthesis was established attention was turned to the isola— tion and purification of enzymes of car...

Enzymatic synthesis of carotenes and related compounds.

Data are presented in this paper which establish many of the reactions involved in the biosynthesis of carotenes. Studies have shown that all of the enzymes required for the synthesis of acyclic and cyclic carotenes from mevalonic acid are present in plastids of tomato fruits. Thus, it has been demonstrated that a soluble extract of an acetone powder of tomato fruits converts mevalonic acid to ...

Carotenes and retinal in Phycomyces mutants.

Three different types of beta-carotene mutants of Phycomyces have been studied. In 2 mutants (Type I) beta-carotene is still the principal carotene but scaled down or up relative to wild type. The carotene mixture of 2 mutants (Type II) consists mainly of phytoene and phytofluene. In Type III (2 mutants) beta-carotene is replaced by lycopene. The examination of the mutants reveals that the rece...

Determination of carotenes by thin-layer chromatography.

The carotenoids of yellow, red and tangerine tomato fruits were separated and identified by thin-layer chromatography (TLC). β-carotene is extracted with a mixture content hexan and acetone. The various systems of adsorbents and solvents for TLC of carote and tomato carotenes were examined, and it was revealed that the most suitable TLC system for separation and identification was silica gel pl...

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